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KMID : 1237720100430010025
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Anatomy & Cell Biology
2010 Volume.43 No. 1 p.25 ~ p.35
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Vitamin C acts indirectly to modulate isotype switching in mouse B cells
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Woo A-Mi
Kang Jae-Seung
Lee Wang-Jae
Hwang Young-Il
Maeng Hyung-Gun
Lee Yong-Taek
Kim Jin-Hee
Jeong Young-Joo
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Abstract
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Vitamin C, one of essential micronutrients, has been reported to modulate the humoral immune responses in some mammals. We investigated whether vitamin C might modulate this response in mice by directly affecting B cells. Splenic B cells were isolated and activated by CD40- and B cell receptor-ligation in vitro. The cells were cultured with a pretreatment of vitamin C from 0 to 1 mM of concentrations. Vitamin C slightly increased apoptosis of B cells dose-dependently and behaved as an antioxidant. We found that in vivo administration of vitamin C by intraperitoneal injection affected isotype switching as previously reported: the titer of antigen-specific IgG1 antibody was decreased, while that of IgG2a was unaffected. Somewhat different from those observed in vivo,in vitro exposure to vitamin C slightly decreased isotype switching to IgG1 and increased isotype switching to IgG2a. Pretreatment with vitamin C in the safe range did not affect either proliferation of cultured B cells or the expression of CD80 and CD86 in those cells. Taken together, in vivo results suggest that vitamin C acts to modulate isotype switching in the mouse. However, because of our in vitro results, we suggest that the modulation exerted by vitamin C in vivo is by indirectly affecting B cells, perhaps by directly influencing other immune cells such as dendritic cells.
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KEYWORD
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Vitamin C, antioxidant, reactive oxygen species, mouse B cell, isotype switching
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